

" Determining watersheds in digital pictures via flooding simulations".
#MERGE IMAGES IMAGEJ UPDATE#
This brings up a dialog where you can activate additional update sites.ģ) Activate the IJPB-plugins update site and close the dialog. from the Fiji menu to start the updater.Ģ) Click on Manage update sites.
#MERGE IMAGES IMAGEJ INSTALL#
To install it, you just need to add the IJPB-plugins update site:ġ) Select Help › Update. The Classic Watershed plugin is part of the MorphoLibJ library. Watershed segmentation on Gaussian-blurred original image (radius = 3, Min h = 0, Max h = 150) Run("Classic Watershed", "input=blobs-blur mask=None use min=0 max=150") apply LUT to facilitate result visualization Run("Classic Watershed", "input=blobs mask=None use min=0 max=150") invert LUT and pixel values to have dark blobs This short macro runs the plugin twice in the blobs sample, first without pre-processing and then after applying a Gaussian blur of radius 3: Several denoising methods are available in Fiji/ImageJ, namely: median filtering, Gaussian blur, bilateral filtering, etc. In that case, it is recommended to either pre-process the image before running the plugin, or merge regions based on a similarity criterion afterwards. Normally, Classic Watershed will lead to an over-segmentation of the input image, especially for noisy images with many regional minima. Labeled image containing the resulting catchment basins (with integer values 1, 2, 3.) and watershed lines (with 0 value).Max h: maximum grayscale value to reach with flooding (by default, set to the maximum value of the image type).Min h: minimum grayscale value to start flooding from (by default, set to the minimum value of the image type).Use diagonal connectivity: select to allow the flooding in diagonal directions (8-connectivity in 2D and 26-connectivity in 3D).Set to "None" to run the method on the whole input image. Mask image (optional): binary image of the same dimensions as the input image which can be used to restrict the areas of application of the algorithm.Input image: grayscale image to flood, usually the gradient of an image.Main dialog of the Classic Watershed plugin
